This directory contains alignments of the following assemblies:
- target/reference: Zebrafish (danRer4, Mar. 2006, Sanger Centre, Danio rerio Sequencing Project Zv6)
- query: Tetraodon (tetNig1, Feb. 2004, Genoscope and Broad Institute, V7)
Files included in this directory:
- md5sum.txt: md5sum checksums for the files in this directory
- danRer4.tetNig1.all.chain.gz: chained blastz alignments. The chain format is
described in http://genome.ucsc.edu/goldenPath/help/chain.html .
- danRer4.tetNig1.net.gz: "net" file that describes rearrangements between
the species and the best Tetraodon match to any part of the
Zebrafish genome. The net format is described in
http://genome.ucsc.edu/goldenPath/help/net.html .
- axtNet/*.danRer4.tetNig1.net.axt.gz: chained and netted alignments,
i.e. the best chains in the Zebrafish genome, with gaps in the best
chains filled in by next-best chains where possible. The axt format is
described in http://genome.ucsc.edu/goldenPath/help/axt.html .
The danRer4 and tetNig1 assemblies were aligned by the blastz alignment
program, which is available from Webb Miller's lab at Penn State
University (http://www.bx.psu.edu/miller_lab/). Any danRer4 sequences larger
than 505,000 bases were split into chunks of 505,000 bases overlapping
by 5,000 bases for alignment. A similar process was followed for tetNig1,
with chunks of 1000,000,000 overlapping by 0. Following alignment, the
coordinates of the chunk alignments were corrected by the
blastz-normalizeLav script written by Scott Schwartz of Penn State.
The blastz scoring matrix (Q parameter) used was:
A C G T
A 91 -90 -25 -100
C -90 100 -100 -25
G -25 -100 100 -90
T -100 -25 -90 91
with a gap open penalty of O=400 and a gap extension penalty of E=30.
The minimum score for an alignment to be kept was K=3000 for the first pass
and L=2200 for the second pass, which restricted the search space to the
regions between two alignments found in the first pass. The minimum
score for alignments to be interpolated between was H=2500. Other blastz
parameters specifically set for this species pair:
M=50
For Blastz, the query tetNig1 genome was all in one file for dynamic masking
to be effective (M parameter). The file consisted the scaffolds for the
random chromosomes and complete sequences for the other chromosomes.
The .lav format blastz output was translated to the .psl format with
lavToPsl, then chained by the axtChain program.
Chain minimum score: 5000, and linearGap matrix of (loose):
tablesize 11
smallSize 111
position 1 2 3 11 111 2111 12111 32111 72111 152111 252111
qGap 325 360 400 450 600 1100 3600 7600 15600 31600 56600
tGap 325 360 400 450 600 1100 3600 7600 15600 31600 56600
bothGap 625 660 700 750 900 1400 4000 8000 16000 32000 57000
Chained alignments were processed into nets by the chainNet, netSyntenic,
and netClass programs.
Best-chain alignments in axt format were extracted by the netToAxt program.
All programs run after blastz were written by Jim Kent at UCSC.
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If you plan to download a large file or multiple files from this directory,
we recommend you use ftp rather than downloading the files via our website.
To do so, ftp to hgdownload.cse.ucsc.edu, then go to the directory
goldenPath/danRer4/vsTetNig1/. To download multiple files, use the "mget"
command:
mget <filename1> <filename2> ...
- or -
mget -a (to download all files in the current directory)
All files in this directory are freely available for public use.
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References
Chiaromonte, F., Yap, V.B., and Miller, W. Scoring pairwise genomic
sequence alignments. Pac Symp Biocomput 2002, 115-26 (2002).
Kent, W.J., Baertsch, R., Hinrichs, A., Miller, W., and Haussler, D.
Evolution's cauldron: Duplication, deletion, and rearrangement in the
mouse and human genomes. Proc Natl Acad Sci USA 100(20), 11484-11489
(2003).
Schwartz, S., Kent, W.J., Smit, A., Zhang, Z., Baertsch, R., Hardison, R.,
Haussler, D., and Miller, W. Human-mouse alignments with BLASTZ</A>.
Genome Res. 13(1), 103-7 (2003).
Name Last modified Size Description
Parent Directory -
axtNet/ 2006-04-30 11:00 -
md5sum.txt 2006-04-30 11:00 2.1K
danRer4.tetNig1.net.gz 2006-04-30 10:58 20M
danRer4.tetNig1.all.chain.gz 2006-04-29 22:40 46M