This directory contains alignments of the following assemblies:
- target/reference: C. elegans
(ce11, Feb. 2013 (WBcel235/ce11),
C. elegans Sequencing Consortium WBcel235)
- query: Hookworm
(necAme1, Dec. 2013 (WS242/N_americanus_v1/necAme1),
The Genome Institute at Washington University)
Files included in this directory:
- md5sum.txt: md5sum checksums for the files in this directory
- ce11.necAme1.all.chain.gz: chained blastz alignments. The chain format is
described in http://genome.ucsc.edu/goldenPath/help/chain.html .
- ce11.necAme1.net.gz: "net" file that describes rearrangements between
the species and the best Hookworm match to any part of the
C. elegans genome. The net format is described in
http://genome.ucsc.edu/goldenPath/help/net.html .
- axtNet/*.ce11.necAme1.net.axt.gz: chained and netted alignments,
i.e. the best chains in the C. elegans genome, with gaps in the best
chains filled in by next-best chains where possible. The axt format is
described in http://genome.ucsc.edu/goldenPath/help/axt.html .
- mafSynNet/*.maf.gz - filtered net files for syntenic alignments
only, in MAF format, see also, description of MAF format:
http://genome.ucsc.edu/FAQ/FAQformat.html#format5
- reciprocalBest/ directory, contains reciprocal-best netted chains
for ce11-necAme1
The ce11 and necAme1 assemblies were aligned by the blastz alignment
program, which is available from Webb Miller's lab at Penn State
University (http://www.bx.psu.edu/miller_lab/). Any ce11 sequences larger
than 1,010,000 bases were split into chunks of 1,010,000 bases overlapping
by 10,000 bases for alignment. A similar process was followed for necAme1,
with chunks of 1,000,000 overlapping by 0. Following alignment, the
coordinates of the chunk alignments were corrected by the
blastz-normalizeLav script written by Scott Schwartz of Penn State.
The blastz scoring matrix (Q parameter) used was the default matrix:
A C G T
A 91 -114 -31 -123
C -114 100 -125 -31
G -31 -125 100 -114
T -123 -31 -114 91
with a gap open penalty of O=400 and a gap extension penalty of E=30.
The minimum score for an alignment to be kept was K=3000 for the first pass
and L=3000 for the second pass, which restricted the search space to the
regions between two alignments found in the first pass. The minimum
score for alignments to be interpolated between was H=2000. Other blastz
parameters specifically set for this species pair:
M=50
The .lav format blastz output was translated to the .psl format with
lavToPsl, then chained by the axtChain program.
Chain minimum score: 1000, and linearGap matrix of (loose):
tablesize 11
smallSize 111
position 1 2 3 11 111 2111 12111 32111 72111 152111 252111
qGap 325 360 400 450 600 1100 3600 7600 15600 31600 56600
tGap 325 360 400 450 600 1100 3600 7600 15600 31600 56600
bothGap 625 660 700 750 900 1400 4000 8000 16000 32000 57000
Chained alignments were processed into nets by the chainNet, netSyntenic,
and netClass programs.
Best-chain alignments in axt format were extracted by the netToAxt program.
All programs run after blastz were written by Jim Kent at UCSC.
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If you plan to download a large file or multiple files from this directory,
we recommend you use ftp rather than downloading the files via our website.
To do so, ftp to hgdownload.cse.ucsc.edu, then go to the directory
goldenPath/ce11/vsNecAme1/. To download multiple files, use the "mget"
command:
mget <filename1> <filename2> ...
- or -
mget -a (to download all files in the current directory)
All files in this directory are freely available for public use.
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References
Chiaromonte F, Yap VB, Miller W. Scoring pairwise genomic sequence
alignments. Pac Symp Biocomput. 2002:115-26.
Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D.
Evolution's cauldron: Duplication, deletion, and rearrangement in the
mouse and human genomes. Proc Natl Acad Sci U S A. 2003 Sep
30;100(20):11484-9.
Schwartz S, Kent WJ, Smit A, Zhang Z, Baertsch R, Hardison RC,
Haussler D, Miller W. Human-Mouse Alignments with BLASTZ. Genome
Res. 2003 Jan;13(1):103-7.
Name Last modified Size Description
Parent Directory -
axtNet/ 2015-07-23 12:04 -
mafSynNet/ 2015-07-23 12:07 -
reciprocalBest/ 2015-07-23 12:49 -
md5sum.txt 2015-07-23 12:04 608
ce11.necAme1.net.gz 2015-07-23 12:04 1.2M
ce11.necAme1.all.chain.gz 2015-07-23 12:03 1.9M